||Development of method for semivolatile organic priority pollutants in fish /
Engel, Tina M.
||Battelle Columbus Labs., OH.;Environmental Monitoring and Support Lab.-Cincinnati, OH.
|| U.S. Environmental Protection Agency, Research and Development, Environmental Monitoring and Support Laboratory,
Fishes--Effect of water pollution on--United States. ;
Pollutants--United States. ;
Fishes--United States--Effect of water pollution on
Water pollution ;
Gas chromatography ;
Mass spectroscopy ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||89 pages ; 28 cm
A method was developed for the determination of neutral and acidic priority pollutant compounds in fish tissue. Priority pollutant compounds are extracted from the fish tissue during homogenization of the tissue in acetonitrile. The acetonitrile extract is isolated from the tissue after centrifuging and placed in a tumbling bottle containing salted water buffered to pH 4, methyl t-butyl ether (MTBE), and petroleum ether. The mixture is equilibrated by tumbling and a portion of the organic layer is isolated after drying over sodium sulfate. The acetonitrile partitioning step serves to remove a portion of the extractable fish lipid material from the priority pollutant compounds. The organic extract is concentrated and the remaining interfering lipid material is removed using a gel permeation chromatography (GPC) procedure.
Cover title. "December 1985." Microfiche.