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OLS Field Name OLS Field Data
Main Title Anomalies in the Enumeration of Starved Bacteria on Culture Media Containing Nalidixic Acid and Tetracycline.
Author Genthner, F. J. ; Upadhyay, J. ; Campbell, R. P. ; Genthner, B. R. S. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Virginia Polytechnic Inst. and State Univ., Blacksburg. ;Technical Resources, Inc., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/515;
Stock Number PB91-206870
Additional Subjects Pseudomonas fluorescens ; Culture media ; Tetracycline ; Nalidixic acid ; Antibiotic resistance ; Microbial drug resistance ; Genetic engineering ; Catalase ; Starvation ; Microbial colony count ; Reprints ;
Holdings
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Status
NTIS  PB91-206870 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 11/26/1991
Collation 8p
Abstract
Culturable counts of antibiotic resistant, genetically engineered Pseudomonas fluorescens were determined on antibiotic-containing plate count agar during starvation in water. The mean of the colony counts on plate count agar with or without tetracycline (4.9 times ten to the sixth power/ml) was significantly higher than mean colony counts on plate count agar containing either nalidixic acid or nalidixic acid plus tetracycline (2.5 times ten to the sixth power/ml). After 20 days of starvation the highest mean colony counts continued to be obtained on plate count agar (7.2 times ten to the sixth power/ml) with slightly, but significantly, lower counts obtained on plate count agar containing either nalidixic acid (5.6 times ten to the sixth power/ml) or tetracylcline (1.5 times ten to the sixth power/ml). A combination of nalidixic acid and tetracycline in plate count agar, however, dramatically reduced colony counts (8.3 x 100/ml) after the starvation period. The addition of catalase to plate count agar containing nalidixic acid and tetracycline negated the effect caused by the combination.