Record Display for the EPA National Library Catalog
RECORD NUMBER: 26 OF 86
|OLS Field Name||OLS Field Data|
|Main Title||Effects of Ammonium and Nitrate on Nutrient Uptake and Activity of Nitrogen Assimilating Enzymes in Western Hemlock.|
|Author||Knoepp, J. D. ; Turner, D. P. ; Tingey, D. T. ;|
|CORP Author||Southeastern Forest Experiment Station, Otto, NC. ;ManTech Environmental Technology, Inc., Corvallis, OR.;Corvallis Environmental Research Lab., OR.|
|Additional Subjects||Forest trees ; Nutrients ; Aquaculture ; Liquids ; Nitrate reductases ; Ammonium compounds ; Reduction(Chemistry) ; Nutritional value ; Tissues(Biology) ; Roots ; Enzymes ; Ion exchanging ; Potassium ; Reprints ; Tsuga heterophylla ; Western hemlock ; Seedlings ; Nitrogen glutamine dehydrogenase|
Western hemlock seedlings were grown in nutrient solutions with ammonium, nitrate or ammonium plus nitrate as nitrogen sources. The objectives were to examine (1) possible selectivity for ammonium or nitrate as an N source, (2) the maintenance of charge balance during ammonium and nitrate uptake, and (3) the activity of the nitrogen assimilating enzymes, nitrate reductase, glutamine synthetase, and glutamine dehydrogenase, in relation to the uptake of different nitrogen sources. The uptake studies revealed that western hemlock takes up ammonium faster than nitrate and that ammonium partially inhibits nitrate uptake. Nitrate reductase activity varied with nitrate availability in root tissue, but showed no response in needles, indicating that most nitrate is reduced in the roots. Results indicate that western hemlock may be adapted to sites where NH(4+) is the predominate N source.