||Comparison of Short- and Long-Term Sexual Reproduction Tests with the Marine Red Alga 'Champia parvula'.
Thursby, G. E. ;
Steele., R. L. ;
||Rhode Island Univ., Kingston. Dept. of Botany.;Environmental Research Lab., Narragansett, RI.
||EPA/600/J-86/518 ; ERLN-785
Water pollution ;
Champia parvula ;
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A 2-d exposure test using the marine red alga Champia parvula was developed to assess the toxic effects of pollutants entering the marine and estuarine environments. The initial exposure was followed by a 5 to 7 day recovery period to allow the development of any cystocarps (evidence of sexual reproduction) the endpoint measured. The 2-d exposure test was validated by comparison with a previously developed 2-week test in which 'no sexual reproduction' was used as the reproductive endpoint. The 2-d procedure is intended for use in testing both single compounds and complex effluents. Complex effluents, however, cannot be accurately tested with the 2-week test; therefore, only single compounds were used to compare the two procedures. Nine single compounds were tested. Concentrations that resulted in a 95% or greater decrease from the control response with the 2-d exposure were considered the best estimate of the no sexual reproduction response from the 2-week test. All of the single compounds tested, except cadmium, yielded essentially the same results with both tests.