||Utilization of the Rabbit Alveolar Macrophage and Chinese Hamster Ovary Cell for Evaluation of the Toxicity of Particulate Materials. II. Particles from Coal-Related Processes.
Garrett, Neil E. ;
Campbell, James A. ;
Stack, H. Frank ;
Waters, Michael D. ;
Lewtas, Joellen ;
||Health Effects Research Lab., Research Triangle Park, NC.
Coal gasification ;
In vitro analysis ;
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Rabbit alveolar macrophage (RAM) and Chinese hamster ovary (CHO) cells were used in vitro tests to evaluate the toxicity of particulate effluents from coal gasification, fluidized-bed combustion, and conventional coal combustion. Evaluation of the cytotoxicity of nine samples from coal energy-related processes showed that the sensitivity of the RAM assay was improved substantially when the test was conducted in serum-free media. A linear relationship was observed between percentage cell viability and ATP level in the particle-treated cultures.