Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Report on Vitellogenin Gene Expression in Fathead Minnows and Pearl Dace from Reference (Non-Dosed) and Lakes Dosed with EE2 in the Canadian Experimental Lakes Area.
Author Flick, R. W. ; Lazorchak, J. M. ; Smith, M. E. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Office of Research and Development.
Publisher Sep 2004
Year Published 2004
Report Number EPA/600/R-04/173;
Stock Number PB2006-114611
Additional Subjects Fish populations ; Selected species ; Endocrine disruption project ; Ontario (Canada) ; Synthetic estrogen ; Vitellogenin gene expression ; Research project ; Fishes ; Vitellogenin (Vtg)
Library Call Number Additional Info Location Last
NTIS  PB2006-114611 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 05/28/2007
Collation 88p
A whole-lake endocrine disruption experiment was conducted by Fisheries and Oceans Canada at the Experimental Lakes Area (ELA) in northwestern Ontario for three years beginning in 2001. This experiment examined population, organismal, biochemical and cellular-level effects in lake trout, white sucker, fathead minnow, and pearl dace exposed to environmentally-relevant (4-6 ng/L) concentrations of the synthetic estrogen, 17alpha-ethynylestradiol. The USEPA collaborated in this study by evaluating vitellogenin (vtg) gene expression in: (1) indigenous fathead minnows and pearl dace collected from the dosed and reference lakes in 2001 through 2003, before and after dosing; (2) indigenous minnows collected in 2001from the reference lake and deployed for 1, 3, 7 and 13 days in the dosed and reference lakes; and (3) Cincinnati cultured minnows exposed to water collected in 2001 through 2004 from ELA lakes and shipped to Cincinnati. RT-PCR methods were used to measure vtg expression.