||Production of Viable 'Giardia' Cysts in vitro: Determination by Fluorogenic Dye Staining, Excystation, and Animal Infectivity in the Mouse and Mongolian Gerbil (Journal Version).
Schupp, D. G. ;
Januschka, M. M. ;
Sherlock, L. A. F. ;
Stibbs, H. H. ;
Meyer, E. A. ;
||Minnesota Univ., Minneapolis. ;Oregon Univ. Health Sciences Center, Portland. Dept. of Microbiology and Immunology. ;Washington Univ., Seattle. School of Public Health and Community Medicine.;Health Effects Research Lab., Research Triangle Park, NC.
Parasitic intestinal diseases ;
Cultured cells ;
Electron microscopy ;
Laboratory animals ;
Life cycle ;
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The purpose of the research was to document the formation of viable Giardia cysts in vitro. Viability staining, using fluorogenic dyes that required metabolic conversion for detection, and immunocytochemistry at the light microscopic level, provided information on viability as well as the identification of antigens that were similar to those found with in vitro formed cysts. Analysis of cysts formed in vivo and in vitro showed similar morphological appearances by both light and electron microscopy. Cysts formed in vitro were capable of establishing infections in both the mouse and gerbil models for giardiasis. Trophozoites recovered from mice experimentally infected with cysts produced in vitro could be maintained in culture and induced a second time to form cysts in vitro. The model, for the production of viable Giardia cysts in vitro, should facilitate research on controlling the complete life-cycle of Giardia outside an animal host. (Copyright (c) 1988 by the American Gastroenterological Association.)