Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Photo-Oxidation of Automobile Emissions: Measurements of the Transformation Products and Their Mutagenic Activity.
Author Kleindienst, T. E. ; Smith, D. F. ; Hudgens, E. E. ; Snow, R. F. ; Perry, E. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/428;
Stock Number PB93-141273
Additional Subjects Exhaust emissions ; Air pollution sampling ; Biological effects ; Photochemical reactions ; Oxidation ; Mutagens ; Automobiles ; Smog ; Mobile pollutant sources ; Mutagen screening ; Toxicity ; Concentration(Composition) ; Bacteria ; Public health ; Bioassay ; Environmental health ; Reprints ; Chemical reaction mechanisms ; Salmonella typhimurium
Library Call Number Additional Info Location Last
NTIS  PB93-141273 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/12/1993
Collation 15p
Dilute mixtures of automobile emissions were irradiated in a smog chamber and tested for mutagenic activity by using a variant of the Salmonella test. The exhaust was taken from single vehicle, a 1977 Ford Mustang, equipped with a catalytic converter. Irradiated and nonirradiate gas-phase emissions were used in exposures of the bacteria, Salmonella typhimurium. The mutagenic activities of extracts from the particulate phase were also measured with the standard plate incorporation assay. (In all experiments only direct-acting mutagenic compounds were measured). The gas-phase data showed increased activity for the irradiated emissions when compared to the nonirradiated mixture, which exhibited negligible activity with respect to the control values. The particulate phase for both the irradiated and nonirradiated mixtures showed negligible activity when results were compared to control values for both strains. However, the experimental conditions limited the amount of extractable mass which could be collected in the particulate phase. Under the experimental conditions of this study, the mutagenic density of the gas-phase component of the irradiated mixture contributed approximately two orders of magnitude more to the total TA100 activity than did the particulate phase. For TA98, the gas-phase component contributed approximately one order of magnitude more. The significance and limitations of the results are discussed.