||Temporal Allocation of (14)C to Extramatrical Hyphae of Ectomycorrhizal Ponderosa Pine Seedlings.
Miller, S. L. ;
Durall, D. M. ;
Rygiewicz, P. T. ;
||Oregon State Univ., Corvallis.;National Science Foundation, Washington, DC.;Forest Service, Washington, DC.;Corvallis Environmental Research Lab., OR.
||NSF-BSR85-05792 ;NSF-BSR85-05975; EPA/600/J-89/113;
Pine trees ;
Carbon 14 ;
Time dependence ;
Plant anatomy ;
Pinus ponderosa laws ;
Hebeloma crustuliniforme ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Ponderosa pine seedlings were inoculated with Hebeloma crustuliniforme either in growth pouches before they were transplanted to root-mycocosms (P seedlings), or at the time of transfer to root-mycocosms (V seedlings). Uninoculated seedlings served as controls (U seedlings). The use of root-mycocosms allowed examination of portions of hyphae separate from roots and rooting substrate but still in symbiosis with the host. The results thus provided a quantitative basis for estimating hyphal mass and carbon allocation to extramatrical hyphae. The amount of 14CO2 fixed after a 2-h exposure was greatest for P seedlings and least for uninoculated seedlings. Four and nine days after exposure, 14C content was greatest in uninoculated seedlings and least in inoculated seedlings. In isotope distribution and dry mass accumulation, V seedlings were more similar to U than to P seedlings. Calculated on a dry weight basis, the allocation of isotope to mycelium suggested that extramatrical hyphae of P seedlings were a stronger sink for carbon than extramatrical hyphae of V seedlings. (Copyright (c) 1989 Heron Publishing, Victoria, Canada.)