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RECORD NUMBER: 46 OF 73

OLS Field Name OLS Field Data
Main Title Induction of Skin Papillomas in the Sencar Mouse as a Tier 2 Carcinogenesis Bioassay.
Author Bull, R. J. ; Robinson, M. ; Glass, J. ; Cmehil, D. ; Orthoefer, J. ;
CORP Author Health Effects Research Lab., Cincinnati, OH.
Year Published 1981
Report Number EPA-600/J-81-189;
Stock Number PB81-212128
Additional Subjects Bioassay ; Carcinogens ; Toxicology ; In vitro analysis ; In vivo analysis ; Mice ; Skin(Anatomy) ; Pathology ; Reprints ; Carcinogenesis ; Papillomas ; Toxic substances ; NTISEPAORD
Holdings
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Status
NTIS  PB81-212128 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 6p
Abstract
The Toxic Substances Control Act mandates the testing of industrial chemicals for which insufficient evidence of safety exists. One of the more critical areas in chemical carcinogenesis testing is a dependable approach to confirmatory tests (tier 2) of identified positives at a screening level (tier 1). The present work was undertaken as part of a systematic evaluation of a series of short-term in vitro and in vivo tests which possess properties thought desirable for tier 2 testing. Experiments were designed to determine whether the mouse skin responds to initiating doses of carcinogens selected from the major carcinogen classes administered by four routes, oral, IP, Sub Q and topical. Experiments to date include testing of diethylnitrosamine, urethane, benzene, benzo(a)pyrene, DMBA, aflatoxin B1, methylmethane sulfonate, azobenzene, FANFT and lead acetate. Two weeks following application of the initiating doses of each carcinogen a 20 week promotion schedule involving the application of lug of 12-0-tetradecanoyl phorbol-13-acetate (TPA) was initiated. On the basis of preliminary data it is apparent that papilloma development in response to chemical carcinogens varies with route of exposure. For example, it is apparent that the response to urethane administered orally exceeds that observed by the topical route by 3-fold. Conversely, the response of the mouse skin to benzo(a)pyrene is more than 10-fold by the topical vs. systemic routes of exposure. Route specific responses to DMBA, benzene, diethylnitrosamine and aflatoxin B1 are also apparent.