Record Display for the EPA National Library Catalog

RECORD NUMBER: 14 OF 17

OLS Field Name OLS Field Data
Main Title Rapid Glutamate Decarboxylase Assay for Detection of 'Escherichia coli'.
Author Rice, E. W. ; Johnson, C. H. ; Dunnigan, M. E. ; Reasoner, D. J. ;
CORP Author Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. ;Cincinnati Univ., OH. Coll. of Medicine.
Publisher 1993
Year Published 1993
Report Number EPA/600/J-93/496;
Stock Number PB94-130143
Additional Subjects Escherichia coli ; Test methods ; Bioassay ; Glutamates ; Drinking water ; Enzymes ; Decarboxylation ; Culture media ; Sampling ; Cells(Biology) ; Exposure ; Chlorine ; Glutamate decarboxylase
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB94-130143 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 05/14/1994
Collation 5p
Abstract
A rapid test procedure for the enzyme glutamate decarboxylase was developed for the detection of Escherichia coli. The assay procedure was able to confirm the presence of E. coli in enteric broth cultures with a 95 percent specificity for both pure cultures and environmental samples. The procedure was capable of detecting survivors of chlorine exposed cells and was comparable to EC-MUG medium in the multiple tube fermentation procedure.