Record Display for the EPA National Library Catalog

RECORD NUMBER: 3 OF 3

OLS Field Name OLS Field Data
Main Title Toxicological Assessment of Biodegraded Pentachlorophenol: Microtox (Trade Name) and Fish Embryos.
Author Middaugh, D. P. ; Resnick, S. M. ; Lantz, S. E. ; Heard, C. S. ; Mueller, J. G. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL. ;SBP Technologies, Inc., Gulf Breeze, FL.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-93/058 ;CONTRIB-777;
Stock Number PB93-168920
Additional Subjects Pesticides ; Toxicology ; Fishes ; Non-mammalian embryo ; Biodeterioration ; Pseudomonas ; Teratogenic compounds ; Water pollution effects(Animals) ; Reprints ; Phenol/pentachloro ; Menidia beryllina
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB93-168920 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 08/23/1993
Collation 8p
Abstract
A Gram-negative bacterium, Pseudomonas sp. strain SR3, was isolated from soil at a former wood treatment plant in north central Florida. The ability of this bacterium to degrade pentachlorophenol (PCP) was confirmed by growing cells in a basal salts medium in which PCP was the only source of carbon and energy. Degradation from a measured concentration of PCP was observed within 120 h of incubation in the presence of PCP-induced cells of Pseudomonas sp. strain SR3. Microtox 5 min EC50 toxicity tests revealed that aqueous solutions of PCP, measured concentrations 39-40 microgram/ml were toxic but that final biodegraded samples, 0.0006 microgram PCP/ml were nontoxic. However, bioassays with embryonic inland silversides, Menidia beryllina, showed that the biodegraded samples were embryotoxic or teratogenic. Results indicate that while biodegradation of PCP was nearly complete, intermediate metabolites of the degradation process or undergraded impurities in PCP were toxic or teratogenic.