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RECORD NUMBER: 2 OF 2

OLS Field Name OLS Field Data
Main Title Comparison of Membrane Filter, Multiple-Fermentation-Tube, and Presence-Absence Techniques for Detecting Total Coliforms in Small Community Water Systems.
Author Jacobs, N. J. ; Zeigler, W. L. ; Reed, F. C. ; Stukel, T. A. ; Rice, E. W. ;
CORP Author Dartmouth Medical School, Hanover, NH. ;Connecticut River Watershed Council, Easthampton, MA.;Environmental Protection Agency, Cincinnati, OH. Water Engineering Research Lab.
Year Published 1986
Report Number EPA/600/J-86/137;
Stock Number PB87-100624
Additional Subjects Bacteria ; Coliforms ; Comparison ; Filters ; Reprints ; Water pollution detection
Holdings
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Status
NTIS  PB87-100624 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 8p
Abstract
Methods for detecting total coliform bacteria in drinking water were compared using 1483 different drinking water samples from 15 small community water systems in Vermont and New Hampshire. The methods included the membrane filter (MF) technique, a ten tube fermentation tube technique (FT) and the presence-absence (P-A) test. Each technique was evaluated using a 100 ml drinking water sample. Of the 1483 samples tested, 336 (23%) contained coliforms as indicated by either one, two, or all three techniques. The FT detected 82%, the P-A detected 88%, and the MF detected 64% of these positives. All techniques simultaneously detected 55% of the positives. Evaluation of the confirmation efficiency of the P-A technique showed 94% of the presumptive positives confirming as coliforms. Thirteen different species of coliforms were identified from the 37 tests where the P-A was positive, but the MF and FT were negative. The P-A test was simple to inoculate and interpret and was considerably more sensitive than the MF and slightly more sensitive than the FT in detecting coliforms in this type of drinking water supply.