||Vapor sampling device for interface with microtox assay for screening toxic industrial chemicals /
Rogers, K. R.
||Environmental Protection Agency, Las Vegas, NV. National Exposure Research Lab. ;Geological Survey, Columbia, MO. Environmental Research Center.
|| U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory, Human Exposure and Atmospheric Sciences Division, Exposure & Dose Research Branch,
Air pollution monitors ;
Data handling ;
Industrial chemicals ;
Vapor sampling devices ;
Toxic industrial chemicals ;
Microtox Omni test ;
Vapor accumulation kinetics
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
||1 CD-ROM : color illustrations, charts (some color) ; 4 3/4 in.
A time-integrated sampling system interfaced with a toxicity-based assay is reported for monitoring volatile toxic industrial chemicals (TICs). Semipermeable membrane devices (SPMDs) using dimethyl sulfoxide (DMSO) as the fill solvent accumulated each of 17 TICs from the vapor phase. Uptake kinetics experiments for one of these compounds (acrolein) indicated that it was significantly sequestered (i.e., 10 percent of the 24 hr maximum) in as little as 10 min and was concentrated by a facto fo over 200 in 24 hr as measured using both mass aod toxicity assays. The effect of each of the TICs on the Microtox bacterial luminescence assay was determined both from a direct assay and a vapor accumulation assay using SPMDs. Microtox EC(sub 50) values (concetrating yielding 50 percent inhibition) were determined for each of the TICs analyzed and ranged from 0.070 parts per million volume (ppmv) for diketene to 322 ppmv for 1,2-dibromoethane. The rank order of the Microtox EC(sub 50) values for each compound measured directly from liquid was simular but not identical to the Apparent (APP) EC (sub 50) values determined frm the vapor accumulation assay.
"EPA/600/R-04-069." "May 2004." Includes bibliographical references (page 21). CD-ROM.