||High-Performance Liquid Chromatography of Nitrophenols with a Swept-Potential Electrochemical Detector.
Scanlon, J. J. ;
Flaquer, P. A. ;
Robinson, G. W. ;
O'Brien, G. E. ;
Sturrock, P. E. ;
||Georgia Inst. of Tech., Atlanta. School of Chemistry.;Environmental Research Lab., Athens, GA.
High resolution ;
Liquid chromatography ;
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The advantages of a swept-potential electrochemical detector are demonstrated by application to the high-performance liquid chromatography (h.p.l.c.) of nitrophenols. The primary advantage of the detector is that an additional dimension of information is obtained which can be used to help identify a chromatographic peak and help to resolve overlapping peaks. The detector retains the selectivity of a differential-pulse detector and yet is general enough to minimize the time for development of new procedures and to detect true unknowns. The h.p.l.c. of nitrophenols was studied on C-18 reverse-phase columns, with methanol-acetate buffer mobile phases. Selectivity factors of the mono-nitrophenols are independent of the composition of the mobile phase from pH 3.25 to 6.00 and from 40 to 55% methanol. The selectivity factors of dinitrophenols are highly dependent on pH. Limits of detection depend on many parameters but are approximately 1 ng injected for the nitrophenols.