||Natural Abundance 13C NMR Spectroscopy of Double-Stranded DNA.
Rill, Randolph L. ;
Hillard, Jr., Peter R. ;
Bailey, J. Terry ;
Levy, George C. ;
||Florida State Univ., Tallahassee. Dept. of Chemistry.;Health Effects Research Lab., Research Triangle Park, NC.
Nuclear magnetic resonance ;
Deoxyribonucleic acids ;
Performance evaluation ;
Isotopic labeling ;
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Although 13C NMR spectroscopy has already proved extremely useful in studies of biopolymers, including t-RNA's, and single-stranded polynucleotides, no successful study of native double-stranded DNA has been reported. This failure is mainly due to extremely unfavorable 13C spin relaxation parameters (very short T2's and long T1's) expected for the long and relatively rigid chains of typical DNA preparations.