Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Effect of Chlorine Treatment on Infectivity of Hepatitis A Virus.
Author Peterson, David A. ; Hurley, Thomas R. ; Hoff, John C. ; Wolfe, Lauren G. ;
CORP Author Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL.;Municipal Environmental Research Lab., Cincinnati, OH.
Year Published 1983
Report Number EPA-600/J-83-001;
Stock Number PB83-163451
Additional Subjects Hepatitis viruses ; Chlorination ; Disinfection ; Viruses ; Treatment ; Monkeys ; Laboratory animals ; Reprints ; Hepatitis A virus
Library Call Number Additional Info Location Last
NTIS  PB83-163451 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 7p
This study examined the effect of chlorine treatment on the infectivity of hepatitis A virus (HAV). Prodromal chimpanzee feces, shown to induce hepatitis in marmosets (Saguinus sp.), was clarified, and the virus was precipitated with 7% polyethylene glycol 6000, harvested and resuspended. The suspension was layered onto 5 to 30% linear sucrose gradients and centrifuged; the fractions containing HAV were dialyzed, and a 1:500,000 dilution of this preparation induced hepatitis and seroconversion in 2 of 4 marmosets. A 1:50 dilution of this preparation served as inoculum. Untreated inoculum induced overt hepatitis and seroconversion in 100% (5 of 5) of marmosets inoculated intramuscularly.