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OLS Field Name OLS Field Data
Main Title The Impact of Food Bioactives on Health in vitro and ex vivo models / [electronic resource] :
Other Authors
Author Title of a Work
Verhoeckx, Kitty.
Cotter, Paul.
López-Expósito, Iván.
Kleiveland, Charlotte.
Lea, Tor.
Mackie, Alan.
Requena, Teresa.
Swiatecka, Dominika.
Wichers, Harry.
Publisher Springer International Publishing : Imprint: Springer,
Year Published 2015
Call Number ########
ISBN 9783319161044
Subjects Life sciences. ; Human physiology. ; Food science.
Internet Access
Description Access URL
Collation XVII, 338 p. 57 illus., 35 illus. in color. online resource.
Due to license restrictions, this resource is available to EPA employees and authorized contractors only
Contents Notes
Introduction -- Gastrointestinal digestion models, general introduction -- Static digestion models general introduction -- InfoGest consensus method -- Approaches to static digestion models -- Dynamic digestion models general introduction -- The TNO gastro-Intestinal Model (TIM) -- Dynamic Gastric Model (DGM) -- Human Gastric Simulator (Riddet model) -- The DIDGI® System -- General introduction to cells, cell lines and cell culture -- Epithelial cell models; General introduction -- Caco-2 cell line -- HT29 cell line -- The IPEC-J2 cell line -- Co-cultivation of Caco-2 and HT-29MT -- Innate and adaptive immune cells; General introduction -- THP-1 and U937 cells -- Peripheral blood mononuclear cells -- PBMC-derived T cells -- Dendritic Cells -- Co-culture Caco-2/ immune cells -- Enteroendocrine Cell Models: General introduction -- STC-1 cells -- NCI-H716 cells -- Murine GLUTag cells -- In vitro intestinal tissue models: General introduction -- Intestinal crypt organoids as experimental models -- Porcine ex vivo intestinal segment model -- Ussing chamber -- In vitro fermentation models: General Introduction -- One compartment fermentation model -- The TNO in vitro model of the colon - TIM-2 -- The Simulator of the Human Intestinal Microbial Ecosystem - SHIME® -- The computer-controlled multicompartmental dynamic model of the gastrointestinal system SIMGI. This book describes in vitro and ex vivo models that can be employed to investigate effects of digested food products on the GIT, or specific components thereof. Many such models exist and include, for example, those used to study digestion and fermentation in the small and large intestine, to investigate absorption (e.g. Ussing chamber, epithelial cell systems), and to research the immune and enteroendocrine responses (e.g. macrophages, dendritic cells, co-cultures). In each case, these respective assays and models are discussed, and tips and tricks concerning their proper use are given. The Impact of Food Bioactives on Health: In Vitro and Ex Vivo Models is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest. The book highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated, and highlights the strengths and weaknesses of the various assays. It is also an important resource for under-graduate students in the 'food and health' arena.