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RECORD NUMBER: 11 OF 27

OLS Field Name OLS Field Data
Main Title Direct Measurement of Fast Axonal Organelle Transport in the Sciatic Nerve of Rats Treated with Acrylamide.
Author Padilla, S. ; Atkinson, M. B. ; Breuer, A. C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Cleveland Clinic Foundation, OH.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/388;
Stock Number PB95-126553
Additional Subjects Axons ; Organelles ; Sciatic nerve ; Acrylamides ; Toxicology ; Rats ; Dose-response relationships ; Microscopy ; Body weight ; Reprints ;
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB95-126553 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/06/1995
Collation 20p
Abstract
The effects of acrylamide on fast axonal transport have been measured primarily using the indirect methods of isotope or enzyme accumulation. The authors report the first direct evaluation of the effects of subchronic acrylamide dosing (150, 300, or 500 mg/kg total dose, i.e., 50 mg/kg, 2x/wk, for 1.5, 3, 5 wk, respectively) on the fast axonal transport motility machinery itself using video-enhanced differential interference contrast optics with digital image processing and computer analysis. Four principle observations were made: (1) Rapid anterograde transport was not affected at any dosage level within 1 wk after cessation of dosing. (2) A high cumulative dosage (500 mg/kg total) of acrylamide or bisacrylamide produced approximately 7-18% decrease in the rate of retrograde transport in both myelinated and unmyelinated axons. (3) Lower dosages of acrylamide (150 or 300 mg/kg total) produced an increase in retrograde transport rates in myelinated axons only. (4) During the recovery phase for the 500 mg/kg acrylamide animals (i.e., 3 or 5 wk after the last dosage of acrylamide) the rate of anterograde transport in the myelinated axons was decreased at 3 wk but not at 5 wk, and the rate of retrograde transport in the mylinated axons returned to control levels while the retrograde transport in the unmyelinated axons continued at abnormally slow speeds.