Main Title |
Mechanisms of DNA Utilization by Estuarine Microbial Populations. |
Author |
Paul, J. H. ;
DeFlaun, M. F. ;
Jeffrey, W. H. ;
|
CORP Author |
University of South Florida, St. Petersburg. Dept. of Marine Science.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC. |
Publisher |
c1988 |
Year Published |
1988 |
Report Number |
NSF-OCE84-15605; EPA/600/J-89/164; |
Stock Number |
PB90-129495 |
Additional Subjects |
Aquatic microbiology ;
Genetics ;
Deoxyribonucleic acids ;
Ribonucleic acids ;
Plankton ;
Thymidines ;
Reprints ;
Plasmids ;
DNA probes ;
Competitive binding ;
Structural genes ;
Navobiocin ;
Thymine
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB90-129495 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
The mechanisms of utilization of DNA by estarine microbial populations has been investigated by competition experiments and DNA uptake studies. Deoxyribonucleoside monophosphates (dNMP's), thymidine, thymine, and RNA all completed with the uptake of radioactivity from (3H)DNA in 4 hour incubations. In fifteen minute incubations, dNMP's had no effect or stimulated (3H)DNA binding, depending on concentration. Uptake of radioactivity from (3H)DNA resulted in little accumulation of TCA-soluble intracellular radioactivity, and was inhibited by the DNA synthesis inhibitor novobiocin. Molecular fractionation studies indicated that some radioactivity from (3H)DNA appeared in the RNA (10 and 30% at 4 and 24 h respectively) and protein (approximately 3%) fractions. The ability for esturine microbial assemblages to transport gene sequences was investigated by plasmid uptake studies followed by molecular probing. (Copyright (c) 1988 American Society for Microbiology.) |