||Harvesting of Leukocytes from Intestinal Lumen in Murine Giardiasis and Preliminary Characterization of These Cells.
Heyworth, M. F. ;
Owen, R. L. ;
Seaman, W. E. ;
Schaefer, F. W. ;
Jones, A. L. ;
||Health Effects Research Lab., Research Triangle Park, NC. ;Veterans Administration Medical Center, San Francisco, CA. ;California Univ., San Francisco. Dept. of Medicine.
Blood cells ;
Giardia muris ;
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The aims of the study were to develop a method for harvesting leukocytes from the mouse small intestinal lumen and to identify leukocytes which enter the intestinal lumen of mice infected with Giardia muris. Giardia-infected and uninfected BALB/c mice were anesthetized, and the small intestine was removed. The intestinal lumen was then flushed with nutrient medium, and the luminal washings were found to contain substantial numbers of mononuclear leukocytes.