||Detection of Hydroxylated Nitro Aromatic and Hydroxylated Nitro Polycyclic Aromatic Compounds in an Ambient Air Particulate Extract Using Bioassay-Directed Fractionation.
Nishioka, M. G. ;
Howard, C. C. ;
Contos, D. A. ;
Ball, L. M. ;
Lewtas, J. ;
||Battelle Columbus Labs., OH. ;North Carolina Univ. at Chapel Hill. Dept. of Environmental Sciences and Engineering.;Health Effects Research Lab., Research Triangle Park, NC.
Aromatic polycylic hydrocarbons ;
Air pollution ;
Gas chromatography ;
Mass spectroscopy ;
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The report identifies hydroxylated nitro-polynuclear aromatic hydrocarbons (OH-NO2PAHs) in an ambient air particulate extract by electron impact (EI) and negative chemical ionization (NCI) high resolution gas chromatography/mass spectrometry (HRGC/MS) analyses. Bioassay-directed fractionation through three sequential separations led to detection of these OH-NO2-PAHs in a highly mutagenic and substantially simplified subfraction of the extract. The compound class identification was based on comparison of both EI and NCI mass spectra of reference OH-NO2-pyrenes. The HRGC/MC retention values for seven OH-NO2-fluoranthene isomer. Quantification of this compound class by NCI HRGC/MC with on-column injection is demonstrated with sensitivity to 0.5 ng/micro L.