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RECORD NUMBER: 10 OF 25

OLS Field Name OLS Field Data
Main Title Inhibition of Benzo(a)Pyrene-Induced Transformation of C3H/10T1/2 Cells by Allylisopropylacetamide and Isopropylvaleramide.
Author Kuszynski, Charles ; Somogyi, Arpad ; Nesnow, Stephen ; Langenbach, Robert ;
CORP Author Nebraska Univ. Medical Center, Omaha.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1981
Report Number EPA/600/J-80-107;
Stock Number PB82-127564
Additional Subjects Carcinogens ; Drugs ; In vivo analysis ; Inhibitors ; Nitrogen organic compounds ; Cells(Biology) ; Reprints ; Benzopyrenes ; Acetamide/allyl-isopropyl ; Valeramide/isopropyl
Holdings
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Status
NTIS  PB82-127564 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 7p
Abstract
Allylisopropylacetamide (AIA) and isopropylvaleramide (IVA) have been demonstrated previously to protect in vivo against the acute toxicity and adrenal necrotic effect of 7, 12-dimethylbenz(a)anthracene. In the present study, the influence of these two amides on the in vitro transforming ability of two potent carcinogens, benzo(a)pyrene (B(a)P) and 7, 12-dimethylbenz(a)anthracene, on C3H10T1/2 cells was investigated. Both AIA and IVA showed a dose-dependent inhibition of B(a)P-induced transformation of C3H10T1/2 cells when added simultaneously for 24 hr with the carcinogen. While pretreatment, simultaneous treatment, and posttreatment of the cells with AIA or IVA inhibited transformation, the 24-hr post-treatment was somewhat more effective. The protective effect did not appear to result from alterations in B(a)P metabolism inasmuch as aryl hydrocarbon hydroxylase activity and the metabolic products of B(a)P detected by high-pressure liquid chromatography were not changed by AIA or IVA treatment. Furthermore, AIA and IVA did not selectively kill chemically transformed C3H10T1/2 cells, as indicated by the absence of their effect on an established, chemically transformed cell line. AIA and IVA also inhibited 7, 12-dimethylbenz(a)-anthracene-induced transformation of C3H10T1/2 cells. These data suggest that AIA and IVA may be useful protective agents and that they presumably exert their protective effect at some stage between the activation of the carcinogen and the expression of the transformed phenotype.