Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 6

OLS Field Name OLS Field Data
Main Title Long-Term Starvation-Induced Loss of Apparent Antibiotic Resistance in Cells Containing the Plasmid pSa(1+).
Author Griffiths, R. P. ; Moyer, C. L. ; Caldwell, B. A. ; Ye, C. ; Morita, R. Y. ;
CORP Author Oregon State Univ., Corvallis.;Corvallis Environmental Research Lab., OR.
Publisher Jun 89
Year Published 1989
Report Number EPA-R-813413; EPA/600/3-89/059;
Stock Number PB89-214415
Additional Subjects Pseudomonas ; Escherichia coli ; Bacterial proteins ; Starvation ; Plasmids ; Gene expression regulation ; Genetic engineering ; Microbial drug resistance
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB89-214415 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 12/18/1989
Collation 20p
Abstract
Escherichia coli, Pseudomonas fluorescens, and a Pseudomonas sp. strain 133B containing the pSa plasmid were starved in well water for up to 523 days. There were two patterns of apparent antibiotic resistance loss observed. In Pseudomonas sp. strain 133B, there was no apparent loss of antibiotic resistance even after starvation for 340 days. In E. coli, by day 49 there was a ten-fold difference between the number of cells that would grow on antibiotic and non-antibiotic containing plates. By day 523, only 12% of the cells were able to express their antibiotic resistance after they had been first resuscitated on non-antibiotic medium. Cells that could not grow on antibiotic medium even after resuscitation showed a permanent loss of chloramphenicol (Cm) resistance but retained resistance to kanamycin and streptomycin. Restriction enzyme digests show that a region from 13 to 15.5 Kb was deleted. This was essentially the same 2.5 Kb region previously reported as being deleted by spontaneous mutation.