Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Simplified Micro Perchlorination Method for Polychlorinated Biphenyls in Biological Samples.
Author Crist, Howard L. ; Moseman., Robert F. ;
CORP Author Health Effects Research Lab., Research Triangle Park, N.C. Environmental Toxicology Div.
Year Published 1977
Report Number EPA/600/J-77/102;
Stock Number PB-278 379
Additional Subjects Chemical analysis ; Tissues(Biology) ; Milk ; Humans ; Quantitative analysis ; Contaminants ; Toxicology ; Environments ; Reprints ; Analytical methods ; Biphenyl/chloro ; Adipose tissue ; Microanalysis ; Analytical methods ; Toxic substances
Library Call Number Additional Info Location Last
NTIS  PB-278 379 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 7p
A simplified methodology is presented for the micro determination of polychlorinated biphenyls (PCBs) in biological samples, by conversion to the decachlorobiphenyl (DCB) derivative. Beef adipose tissue and human milk extracts were fortified with PCB standards at 0.1-5.0 ppm, and perchlorinated with antimony pentachloride (SbCl5). Several Aroclors representing various degrees of chlorine content were investigated to assess the efficiency of conversion to DCB. Samples were cleaned up on a Florisil mini column and the PCBs were quantitated by electron capture GLC. Several chlorinated pesticides which were subjected to the perchlorination procedure did not interfere. As little as 0.1 ppm PCBs in 500 mg tissue extract can be recovered at 79-99%. The background DCB content of several brands of SbCl5 was determined. The levels of PCBs in human milk obtained by perchlorination technique are compared with data acquired by electron capture gas-liquid chromatography in which the individual chlorobiphenyls in the sample are measured.