||Estimation of the Acetylcholine Levels in Brain Tissue by Gas Chromatography of Acetic Acid.
Cranmer., Morris F. ;
||National Communicable Disease Center, Atlanta, Ga.
Chemical analysis ;
Acetic acid ;
Solvent extraction ;
Gas chromatography ;
Laboratory animals ;
Experimental data ;
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The accurate determination of acetylcholine in mammalian brain is of interest to the pharmacologist studying the effects of organo-phosphate insecticides as well as other agents which may alter normal levels of this amine. The paper describes a method which further simplifies the analysis of acetylcholine by circumventing the reduction step and determining directly the acetic acid derived from acetylcholine.