Main Title |
Cytogenetic Characterization of the L5178Y TK+/-3.7.2C Mouse Lymphoma Cell Line. |
Author |
Sawyer, J. ;
Moore, M. M. ;
Clive, D. ;
Hozier, J. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Florida Inst. of Tech., Melbourne. ;Burroughs Wellcome Co., Research Triangle Park, NC. |
Year Published |
1985 |
Report Number |
EPA/600/J-85/408; |
Stock Number |
PB86-212628 |
Additional Subjects |
Mice ;
Reprints ;
Cell lines ;
Cytogenetics ;
Mouse lymphoma cell lines
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-212628 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
12p |
Abstract |
The cytogenetic characterization of the L5178Y TK+/-3.7.2C mouse lymphoma cell line was carried out, utilizing G-banded metaphase chromosomes, to provide a karyotypic basis for the precise delineation of induced rearrangements in TK-/- mutants. Band-pattern measurements were used to construct ideograms which represent the position, number, size and staining intensity of the chromosome bands. The TK+/- 3.7.2C cell line has been shown to provide quantitation of forward mutations induced at the autosomal thymidine kinase (TK) locus in the cell line. Chromosome analysis of the TK+/-3.7.2C cell line and derived TK-/- mutants has become important in demonstrating that the TK+/- -> TK-/- assay may detect and distinguish between chromosomal events and smaller, perhaps point-mutation, events in mutant colonies. |