Main Title |
Mutagenicity and Clastogenicity of Proflavin in L5178Y/TK(+/-)-3.7.2.C Cells. |
Author |
DeMarini, D. M. ;
Brock, K. H. ;
Doerr, C. L. ;
Moore, M. M. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC. |
Year Published |
1988 |
Report Number |
EPA/600/J-88/017; |
Stock Number |
PB88-219779 |
Additional Subjects |
Mutations ;
Genetic ;
Chromosome abnormalities ;
Reprints ;
Mutagenicity ;
Clastogenicity ;
Proflavin ;
Genetic mutations ;
Biological effects
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB88-219779 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
9p |
Abstract |
The paper evaluated the ability of proflavin to induce specific-locus mutations at the heterozygous thymidine kinase (tk) locus of L5178Y/TK+/- -3.7.2.C mouse lymphoma cells, which permit the recovery and classification of mutants due to single-gene or chromosomal mutation. Proflavin was highly mutagenic at the tk locus, producing 724-965 TK mutants/10(6); survivors. The potent clastogenicity of proflavin was confirmed by cytogenetic analysis for chromosomal aberrations. These results lead to the conclusion that proflavin induces few single-gene mutations in mammalian cells; instead, it is a potent clastogen and produces primarily chromosomal mutations. The authors discuss the possible involvement of DNA topoisomerase II in the clastogenic mechanism of proflavin. |