Record Display for the EPA National Library Catalog

RECORD NUMBER: 27 OF 221

OLS Field Name OLS Field Data
Main Title Changes in In vitro Brain and Spinal Cord Protein Phosphorylation After a Single Oral Administration of Tri-o-Cresyl Phosphate to Hens.
Author Patton, S. E. ; Lapadula, D. M. ; O'Callaghan, J. P. ; Miller, D. B. ; Abou-Donia, M. B. ;
CORP Author Duke Univ. Medical Center, Durham, NC. Dept. of Pharmacology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA/600/J-85/431;
Stock Number PB86-224573
Additional Subjects Toxicology ; Protein ; In vitro analysis ; Brain ; Spinal cord ; Phosphorylation ; Reprints ; Neurotoxicity ; Tri-o-cresyl phosphate
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB86-224573 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 13p
Abstract
The effect of a single oral 750 mg/kg dose of tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of brain and spinal cord proteins was assessed in hens during the development of and recovery from delayed neurotoxicity. Crude membrane and cytosolic fractions were prepared from the brains and spinal cords of control and TOCP-treated hens at 1, 7, 14, 21, 35, and 55 days after treatment. Brain and spinal cord protein phosphorylation with (gamma-32P)ATP was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), autoradiography, and microdensitometry. TOCP administration conferred calcium and calmodulin dependence on the phosphorylation of a few brain cytosolic proteins and caused an increase in the phosphorylation of a number of other cytosolic and membrane proteins.