Record Display for the EPA National Library Catalog

RECORD NUMBER: 39 OF 43

OLS Field Name OLS Field Data
Main Title Primary Cell Cultures from the Teleost, 'Cyprinodon variegatus': Culture Establishment and Application in Carcinogen Exposure Studies.
Author Martin, B. J. ; Ellender, R. D. ; Hillebert, S. A. ; Guess, M. M. ;
CORP Author University of Southern Mississippi, Hattiesburg.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1984
Report Number EPA/600/J-84/271;
Stock Number PB85-193100
Additional Subjects Fishes ; Maintenance ; Laboratory animals ; Reprints ; Carcinogens ; Teratogens ; Cyprinodon variegatus ; Teleost ; Biological assay ; Cultured cells ; Environmental pollution
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB85-193100 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 7p
Abstract
Methods were developed for aseptic maintenance of Cyprinodon variegatus fry for extended periods. Preliminary studies indicated that under optimum conditions sterile embryos develop normally for a sufficient time to function as carcinogenteratogen assay systems. An embryo-primary cell culture technique was developed that incorporates, in a single system, certain characteristics of both intact embryos and primary cell cultures and allows simultaneous observation of the effects of carcinogens on the whole organism and primary cell monolayers. The effective use of these systems provides one the opportunity to study the effects of carcinogens on teleosts at the cellular and organismic level.