Abstract |
The purpose of this study was to evaluate the potential of butyl benzyl phthalate (BBP), administered in the feed to CD(trademark) rats, to produce alterations in parental fertility, maternal pregnancy and lactation, and growth and development of the offspring for two generations, one litter per generation. This study was performed in compliance with U.S. EPA Toxic Substances Control Act (TSCA), Good Laboratory Practice Standards (U.S. EPA, 1989), and the U.S. EPA OPPTS Testing Guidelines (U.S. EPA, 1998). Male and female CD(trademark) (Sprague-Dawley) rats (the F0 generation) were administered BBP (1, 2-Benzenedicarboxylic acid, butyl phenylmethyl ester (9CI); CAS No. 85-68-7) in the feed at 0, 750, 3750, and 11,250 ppm, available ad libitum, 30 animals/sex/dose, for ten weeks. Body weights and feed consumption were recorded weekly, and clinical signs were recorded at least once daily. Vaginal cytology was evaluated for the last three weeks of the prebreed period. Animals were then randomly mated within treatment groups for a two-week mating period to produce the F1 generation, with exposure continuing. |