Main Title |
Quantitation of Small Colony Trifluorothymidine-Resistant Mutants of L5178Y/TK+/- Mouse Lymphoma Cells in RPMI-1640 Medium. |
Author |
Moore, M. M. ;
Howard, B. E. ;
|
CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1982 |
Report Number |
EPA/600/J-82-115; |
Stock Number |
PB84-101252 |
Additional Subjects |
Lymphomas ;
Mutations ;
Mice ;
Cells(Biology) ;
Reprints ;
Thymidine/trifluoro
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB84-101252 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
10p |
Abstract |
The L5178Y/TK t/-mouse lymphoma assay quantitates the induction of thymidine kinase deficient (TK-/-) mutants. When plated in Fischer's medium for leukemic cells of mice, mutants appear morphologically as large and small trifluorothymidine-resistant (TFT) colonies; mutants iolated in Fischer's medium using 1 ug/ml TFT are TK deficient and TFT-resistant upon rechallenge with TFT. Cytogenetic analysis has revealed that mutants represent chromosomal mutants of chromosome 11, whereas mutants appear to represent single gene mutations. A number of laboratories have preferred to use RPMI-1640 medium for the L5178Y/TKt/-assay instead of Fischer's medium. |