||Large Deletions are Tolerated at the 'hprt' Locus of In vivo Derived Human T-Lymphocytes.
Fuscoe, J. C. ;
Zimmerman, L. J. ;
Harrington-Brock, K. ;
Moore, M. M. ;
||Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div. ;Environmental Health Research and Testing, Inc., Research Triangle Park, NC.
Hypoxanthine phosphoribosyltransferase ;
T-lymphocyte gene rearrangement ;
Chromosome deletion ;
Polymerase chain reaction ;
DNA mutational analysis ;
Cultured cells ;
Clone cells ;
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A cloning assay was used to recover hypoxanthine phosphoribosyltransferase (hprt) T-lymphocytes from adult human males. Analysis of crude cellular extracts by polymerase chain reactions (PCRs) demonstrated that 8% (18/218) of the hprt mutations were due to total deletion of the hprt gene. Fourteen of the 18 mutants were examined by PCR for the presence of flanking DNA to determine the extent of the deletions. The largest deletions were greater than 15 times the size of the hprt gene.