Record Display for the EPA National Library Catalog

RECORD NUMBER: 257 OF 271

OLS Field Name OLS Field Data
Main Title Transduction of Linked Chromosomal Genes between 'Pseudomonas aeruginosa' Strains during Incubation In situ in a Freshwater Habitat.
Author Saye, D. J. ; Ogunseitan, O. A. ; Sayler, G. S. ; Miller., R. V. ;
CORP Author Stritch School of Medicine, Maywood, IL. ;Tennessee Univ., Knoxville. Center for Environmental Biotechnology.;Environmental Research Lab., Gulf Breeze, FL.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/512;
Stock Number PB91-199976
Additional Subjects Bacterial genes ; Pseudomonas aeruginosa ; Bacterial chromosomes ; Water microbiology ; Genetic transduction ; Fresh water ; Microbial colony count ; Bacteriophages ; Plasmids ; Genotype ; Reprints ; Lysogens
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB91-199976 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 09/04/1991
Collation 8p
Abstract
Both transduction of single chromosomal loci and cotransduction of closely linked loci were observed between lysogenic and nonlysogenic strains of Pseudomonas aeruginosa in a freshwater habitat. Transductants were recovered at frequencies of 10 to the minus 6 to 10 to the minus 5 transductants per CFU. Transductants of lysogenized strains were recovered 10- to 100-fold more frequently than were transductants of nonlysogenic parents. Lysogens are thus capable of introducing phages which mediate generalized transduction into the natural microbial community and serving as recipients of transduced DNA. It would appear that lysogeny has the potential of increasing the size and flexibility of the gene pool available to natural populations of bacteria. The ability to generate and select new genetic combinations through phage-mediated exchange can be significant in the face of a continually changing environment and may contribute to the apparent fitness of the lysogenic state in natural ecosystems. (Copyright (c) 1990, American Society for Microbiology.)