Record Display for the EPA National Library Catalog
RECORD NUMBER: 170 OF 271
|OLS Field Name||OLS Field Data|
|Main Title||Molecular Characterization of 'Pseudomonas aeruginosa' Bacteriophages: Identification and Characterization of the Novel Virus B86.|
|Author||Kilbane, J. J. ; Miller, R. V. ;|
|CORP Author||Loyola Univ. of Chicago, IL. ;Stritch School of Medicine, Maywood, IL.;Environmental Research Lab., Gulf Breeze, FL.|
|Additional Subjects||Bacteriophages ; Viruses ; Identifying ; Classifications ; Deoxyribonucleic acids ; Immunology ; Virology ; Tests ; Growth ; Culture media ; Morphology ; Isolation ; Enzymes ; Sensitivity ; Electron microscopes ; Reprints ; Pseudomonas aeruginosa ; Lysogens|
The authors have characterized a new phage, B86, of Pseudomonas aeruginosa isolated from nature. It is a temperate, uv-inducible, generalized transducing phage. To determine the relatedness of his phage to other characterized P. aeruginosa phages, DNA homology studies were carried out. P. aeruginosa phages have previously been grouped by immunological cross-reactivity. The studies confirm the classification by demonstrating that phages of different class share little or no DNA homology. Based on homology studies as well as cross-immunity to superinfection, B86 is related to other class B phages and is most homologous with phage B39. The virion morphology of these two phages is quite different, however, as are the restriction enzyme digestion patterns of their genomes with several restrictions enzymes.