Record Display for the EPA National Library Catalog

RECORD NUMBER: 9 OF 19

OLS Field Name OLS Field Data
Main Title Identification of mutagenic components in wastewater effluents and sludges /
Author Tabor, M. Wilson. ; Loper, J. C.
Other Authors
Author Title of a Work
Loper, John C.
CORP Author Cincinnati Univ. Medical Center, OH.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory,
Year Published 1989
Report Number EPA/600/1-89/007; EPA-R-810792
Stock Number PB90-145731
Additional Subjects Sewage treatment ; Waste water ; Municipalities ; Mutagens ; Identifying ; Salmonella ; Bioassay ; Assessments ; Sludge disposal ; Residues ; Drinking water
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-145731 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation 254 pages ; 28 cm
Abstract
Both industrially-impacted and domestic municipal sewage treatment plant wastewaters and sludges have been studied to isolate the residue organics for the characterization of their mutagenic properties and for the isolation/identification of the mutagenic components. Methods were investigated to isolate the residue organics for mutagenic assessment via the Salmonella/microsomal activation, i.e., 59, assay. Residue organics were isolated from wastewaters by a modification of the USEPA/EMSL-LV interim protocol for the isolation of residue organics from drinking water for mutagenic assessment. Residue organics were isolated from sludges via three methods: the Soxhlet extraction method of Hites; the USEPA/EMSL-CIN Method 624S/6255; and a newly-developed method in the study that features the milling of the sludge with anhydrous sodium sulfate to a homogeneous powder followed by sequential extractions using a solvent series from non-polar to polar. The residue organics from wastewaters and sludges were fractionated by high performance liquid chromatography (HPLC) using a coupled bioassay/analytical fractionation approach. In general, mutagenic assessment of the residue organics from the industrially-impacted treatment plant wastewaters and sludges show both direct acting and 59 dependent TA98 mutagenic activity.
Notes
Caption title. "November 1989." "EPA/600/1-89/007." "M. Kate Smith, project officer." "CR-810792." Microfiche.