Record Display for the EPA National Library Catalog

RECORD NUMBER: 17 OF 19

OLS Field Name OLS Field Data
Main Title Phylogenetic Analysis of a Bacterial Aerobic Degrader of Azo Dyes.
Author Govindaswami, M. ; Schmidt, T. M. ; White, D. C. ; Loper, J. C. ;
CORP Author Cincinnati Univ. Medical Center, OH. Dept. of Molecular Genetics, Biochemistry and Microbiology. ;Miami Univ., Oxford, OH. Dept. of Microbiology. ;Tennessee Univ., Knoxville. Center for Environmental Biotechnology.;Environmental Protection Agency, Gulf Breeze, FL. Gulf Breeze Environmental Research Lab.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/236;
Stock Number PB94-170081
Additional Subjects Azo dyes ; Biodeterioration ; Aerobic bacteria ; Phylogeny ; Ribosomal RNA ; Molecular cloning ; Polymerase chain reaction ; DNA sequence analysis ; Fatty acids. Reprints ; Caulobacter subvibrioides
Holdings
Library Call Number Additional Info Location Last
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Status
NTIS  PB94-170081 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/01/1994
Collation 7p
Abstract
Eubacterial consensus obligonucleotide primers were used to amplify by polymerase chain reaction the nearly full-length 16S rRNA gene of isolate C7, a gram-negative rod capable of aerobic degradation of azo dyes. The DNA product was cloned and sequenced. Phylogenetic analysis based upon this DNA sequence places C7 within the alpha subdivision of proteobacteria, most closely related to Caulobacter subvibrioides. The phospholipid fatty acid pattern resembles that of caulobacters, with monounsaturated 16- and 18-carbon fatty acids predominating. C7 is unusual in having a monounsaturated branched fatty acid in the phospholipids and exclusively 2-hydroxy fatty acids in the lipid-extracted residue. This organism is of potential use in bioreactors operated for azo dye degradation. (Copyright (c) 1993, American Society for Microbiology.)