Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Mutagen Isolation Methods: Fractionation of Residue Organics from Aqueous Environmental Samples.
Author Tabor, M. W. ; Loper, J. C. ;
CORP Author Cincinnati Univ. Medical Center, OH. Inst. of Environmental Health.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1985
Report Number EPA-R-810792; EPA/600/D-85/062;
Stock Number PB85-218865
Additional Subjects Mutagens ; Organic compounds ; Water pollution ; Tests ; Salmonella ; Toxicity ; Distillation ; Bioassay ; Disinfection ; Water treatment ; Residues ; Separation ;
Library Call Number Additional Info Location Last
NTIS  PB85-218865 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/21/1988
Collation 34p
A basic assessment of the mutagenic activity of organic residues from water entails application of the Salmonella test to both the parent residue mixture and to major subfractions obtained by reverse phase and/or normal phase HPLC. Such analyses overcome the presence in particular residue mixtures of components which are antagonistic or toxic for the bioassay. When applied to residues obtained by various procedures, the method reveals a wide range of mutagenic activity among environmental waters, depending upon the source of the water sample, the apparent types of natural and anthropogenic compounds it contains, and the water processing and disinfection procedures used in its treatment. Through combinations of specific mutagenic bioassays plus sequential analytical and preparative HPLC fractionations, the method has been shown to be applicable for the isolation of mutagens from residues of a variety of environmental waters.