Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title DNA (Deoxyribonucleic Acid) Adsorption to Soils and Sediments.
Author Ogram, A. ; Sayler, G. S. ; Gustin, D. ; Lewis, R. J. ;
CORP Author Tennessee Univ., Knoxville.;Environmental Research Lab., Gulf Breeze, FL.;National Institutes of Health, Bethesda, MD.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-89/038;
Stock Number PB90-103573
Additional Subjects Sediments ; Absorption ; Deoxyribonucleic acids ; Soil chemistry ; Ionic strength ; PH ; Extraction ; Montmorillonite ; Sands ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB90-103573 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/10/1990
Collation 5p
Deoxyribonucleic acid (DNA) adsorption of five soils, an acid-washed sand, and a lake sediment was investigated. All DNA at environmentally relevant concentrations was adsorbed by soils containing a significant amount of montmorillonite at low to neutral pH values. Studies on the effects of DNA molecular size on adsorption to sand and a sandy soil were described by the Freundlich isotherm model (r2 >0.85), and revealed that the higher the molecular weight, the more the adsorption. The effects of ionic strength (as sodium phosphate buffer) on adsorption showed that adsorption decreases as PO4 increases. Organic carbon was found to play a relatively minor role in the adsorption of DNA to these soils. A scheme for the extraction of DNA from soils was also developed. (Copyright 1988 American Chemical Society.)