Record Display for the EPA National Library Catalog

RECORD NUMBER: 17 OF 18

OLS Field Name OLS Field Data
Main Title Toxic Interactions between Carbon Tetrachloride and Chloroform in Cultured Rat Hepatocytes.
Author Lamb, R. G. ; Borzelleca, J. F. ; Condie, L. W. ; Gennings, C. ;
CORP Author Virginia Commonwealth Univ., Richmond. Dept. of Pharmacology and Toxicology.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA-R812558; EPA/600/J-89/330;
Stock Number PB90-216052
Additional Subjects Carbon tetrachloride ; Chloroform ; Toxicology ; Liver ; Rats ; Cholines ; Graphs(Charts) ; Tables(Data) ; Reprints ; Phosphatidylcholines ; Dose-response relationships ; Drug interactions ; Cultured cells ; Cell survival
Holdings
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Status
NTIS  PB90-216052 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 08/27/1990
Collation 10p
Abstract
Primary cultures of adult rat hepatocytes were incubated (1.5-16 hr) with various concentrations of CCl4(< or = 0.5 mM) and/or CHCl3(< or = 2.5 mM). Agent-dependent alterations in hepatocyte functions were assessed by measuring (1) (3H)choline incorporation into phosphatidylcholine (endoplasmic reticulum), (2) MTT (tetrazolium salt) reduction (mitochondria), and (3) AST release into medium (plasma membrane). Cultured hepatocytes incubated with 0.5 mM CCl4 displayed a significant (p < or = 0.001) and rapid (1.5 hr) reduction (40%) in endoplasmic reticulum function that preceded significant (p < or = 0.001) alterations in mitochondria (6-16 hr) and plasma membrane (6-16 hr) functions. CCl4-dependent alterations in liver cell functions are a result of CCl4 bioactivation since metyrapone inhibits the CCl4-mediated changes in cell functions. Response surface methods (RSM) were used to determine the influence of combinations of CCl4 and CHCl3 on liver cell MTT reduction and (3H)choline incorporation. Regression coefficients were determined for CCl4, CHCl3, and CCl4-CHCl3. All results were significant (p < 0.0001) and implied that CCl4 was a more potent hepatotoxin in vitro than CHCl3. The RSM analysis also suggested that combinations of CHCl3 and CCl4 have greater than additive effects on MTT reduction and (3H)choline incorporation. (Copyright (c) 1989 by Academic Press, Inc.)