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OLS Field Name OLS Field Data
Main Title Induction of Specific Proteins in Eukaryotic Algae Grown under Iron-, Phosphorus-, or Nitrogen-Deficient Conditions.
Author La Roche, J. ; Geider, R. J. ; Graziano, L. M. ; Murray, H. ; Lewis, K. ;
CORP Author Brookhaven National Lab., Upton, NY. ;Delaware Univ., Lewes. Coll. of Marine Studies.;Environmental Research Lab., Narragansett, RI.
Publisher c1993
Year Published 1993
Report Number EPA/600/J-94/503 ; ERLN-X218
Stock Number PB95-148656
Additional Subjects Proteins ; Algae ; Deficient elements ; Diatoms ; Antibodies ; Growth ; Phytoplankton ; Iron ; Phosphorus ; Nitrogen ; Marine microorganisms ; Induction ; Tracer techniques ; Chlorophyta ; Reprints ; Eukaryotic algae ; Flavodoxin ; Phaeodactylum tricornutum ; Dunaliella tertiolecta ; Bacillariophyceae ; Chaetoceros gracilis
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NTIS  PB95-148656 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 03/06/1995
Collation 13p
Abstract
The document is investigating the possibility that antibodies to proteins repressed by a specific nutrient can be used as probes to indicate which nutrient limits photosynthetic carbon fixation in the ocean. The diatom Phaeodactylum tricornutum Bohlin and the chlorophyte Dunaliella tertiolecta Butcher were grown in batch cultures in artificial seawater and f/2 nutrient lacking either phosphorus, iron, or nitrogen. Chlorosis was inducted by nutrient limitation in both species with the exception of phsophorus-limited D. tertiolecta. The synthesis and appearance of specific proteins were followed by labeling with (14)C-bicarbonate.