||Characterization of an Axenic Strain of 'Hartmannella vermiformis' Obtained from an Investigation of Nosocomial Legionellosis.
Fields, B. S. ;
Nerad, T. A. ;
Sawyer, T. K. ;
King, C. H. ;
Barbaree, J. M. ;
||Center for Infectious Diseases, Atlanta, GA. Div. of Bacterial Diseases. ;American Type Culture Collection, Rockville, MD. ;Rescon Associates, Inc., Royal Oak, MD. ;Georgia Univ., Athens. Coll. of Veterinary Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Cross infections ;
Water microbiology ;
Polyacrylamide gel electrophoresis ;
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A free living amoeba identified as Hartmannella vermiformis was isolated from a water sample obtained during an investigation of nosocomial legionellosis. Hartmannella vermiformis are known to support the intracellular multiplication of Legionella pneumophila. This strain of H. vermiformis, designed CDC-19, was cloned and established in axenic culture, in order to develop a model for the study of the pathogenicity of legionellae. Isoenzyme patterns of axenically cultivated strain CDC-19 were compared with 2 strains of H. vermiformis derived from the type strain, one axenic (ATCC 50236) and the other grown in the presence of bacteria (ATCC 30966). Enzyme patterns suggested that all 3 strains are assignable to the species Hartmannella vermiformis. Axenic H. vermiformis strain CDC-19 has been deposited with the American Type Culture Collection (ATCC 50237) and should prove useful in the study of protozoan-bacterial interaction. (Copyright (c) 1990 American Society of Protozoologists.)