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RECORD NUMBER: 65 OF 123

OLS Field Name OLS Field Data
Main Title Metabolism of 1-Nitropyrene by Human, Rat, and Mouse Intestinal Flora: Mutagenicity of Isolated Metabolites by Direct Analysis of HPLC Fractions with a Microsuspension Reverse Mutation Assay.
Author King, L. C. ; Kohan, M. J. ; George, S. E. ; Lewtas, J. ; Claxton, L. D. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1990
Year Published 1990
Report Number EPA/600/J-90/423;
Stock Number PB91-171785
Additional Subjects Metabolism ; Enterobacteriaceae ; Toxicology ; High pressure liquid chromatography ; Mutagenicity tests ; Aromatic polycyclic hydrocarbons ; Rats ; Human ; Mice ; Reprints ; 1-Nitropyrene
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB91-171785 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 09/04/1991
Collation 16p
Abstract
Among the nitro-substituted polycyclic aromatic hydrocarbons identified in environmental samples and known to be genotoxic, 1-nitropyrene is one of the most abundant. The biotransformation of 1-nitro((14)C)pyrene by human, rat, and mouse intestinal microflora and the mutagenicity of the isolated metabolites by direct analysis of the HPLC fractions with a microsuspension mutation assay were investigated. 1-nitro((14)C)pyrene was metabolized by human, rat and mouse intestinal microflora to the following reductive metabolites; 1-aminopyrene, Nacetylaminopyrene, N-formyl-1-aminopyrene and two unknown metabolites identified as A and B. The predominant metabolite of 1-nitro((14)C)pyrene produced by human, rat or mouse intestinal microflora following a 12 h incubation was 1-aminopyrene which accounted for 79 to 93% of the total (14)C respectively. Only minor amounts of N-formyl-1-aminopyrene (1%), N-acetylaminopyrene (3 - 4%) were produced. The similarity in the distribution of the reductive metabolites suggests that a similar mechanism exists in the biotransformation of 1-nitropyrene by intestinal microflora of different mammalian species.