Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Investigation of Enzymatic Screening Tests for Mutagens in Environmental Pollutants from Synfuel Operations.
Author Schmidt-Collerus, J. J. ; Couse, N. L. ; King, J. ; Leffler, L. ;
CORP Author Denver Research Inst., CO.;Environmental Research Lab., Gulf Breeze, FL.
Year Published 1981
Report Number EPA-R-805671; EPA-600/4-81-038 ; ERL.GB-150
Stock Number PB81-209579
Additional Subjects Mutagens ; Carcinogens ; Screening ; In vitro analysis ; Enzymes ; Biphenyl ; Liquid chromatography ; Terphenyls ; Protozoa ; Water pollution ; Marine biology ; Estuaries ; Synthetic fuels ; Metabolites ; Parauronema acutum ; Water pollution effects(Animals) ; Ecosystems ; NTISEPAORD
Library Call Number Additional Info Location Last
NTIS  PB81-209579 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 06/23/1988
Collation 90p
The objective of this research program was to develop an enzymatic screen for chemical carcinogens based on the selective in vitro stimulation of microsomal biphenyl-2-hydroxylase by known chemical carcinogens. An attempt was made to repeat published work using a spectrophotofluorometric assay for biphenyl metabolities. It was found that this assay system is not valid for use with complex mixtures, and that metabolites must be separated from interfering compounds prior to quantitation. A high pressure liquid chromatography method was developed which permitted rapid separation of metabolites. Nanogram quantities of metabolites were detectable using this chromatographic separation in conjunction with a spectrophotofluorometric detector. Using this method, it was not possible to demonstrate in vitro stimulation of biphenyl-2-hydroxylase by chemical carcinogens. Alternative assays were also examined. Terphenyl is metabolized to at least three different compounds by hamster microsomes. Further work is necessary to validate the utility of this substrate in an enzymatic screen for carcinogens. A marine protozoan, Parauronema acutum metabolizes biphenyl in vivo to 2- and 4-hydroxybiphenyl. This organism may provide a reliable, inexpensive source of biphenyl hydroxylase for an in vitro enzymatic assay system.