Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 17

OLS Field Name OLS Field Data
Main Title Ca(2+)/Calmodulin-Dependent Protein Kinase Enriched in Cerebellar Granule Cells: Identification of a Novel Neuronal Calmodulin-Dependent Protein Kinase.
Author Ohmstede, C. A. ; Jensen, K. F. ; Sahyoun, N. E. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Burroughs Wellcome Co., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA/600/J-89/046;
Stock Number PB90-103623
Additional Subjects Calcium ; Rats ; Genetics ; Chemical analysis ; Isolation ; Reprints ; Protein kinases ; Calmodulin ; Brain chemistry ; Molecular cloning ; Immunohistochemistry ; Monoclonal antibodies ; Poly A ; Base sequence ; Immunoblotting
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-103623 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 12p
Abstract
The authors cloned a novel, brain-specific, Ca/calmodulin-dependent protein kinase from a rat lambda gt11 library which was probed for calmodulin-binding proteins. Antibodies raised against the b-galactosidase fusion protein recognized Mr 65000 and Mr 65000/67000 polypeptides from rat forebrain and cerebellum, respectively. Both polypeptides co-purified to homogeneity and phosphorylated synapsin I. The protein kinase is coded for by two poly A+RNAs at 2.0 kb and 3.5 kb which probably derive from a single gene. A 1.3 kbp cDNA insert contained 477 bp of protein coding sequence which included a calmodulin-binding domain, but no further sequence similarities to other protein kinases. Immunohistochemical localization with a monospecific antibody revealed that the enzyme was expressed in specific subpopulations and was exceptionally enriched in the granule cells of the cerebellum.