Record Display for the EPA National Library Catalog

RECORD NUMBER: 4 OF 7

OLS Field Name OLS Field Data
Main Title Mechanisms of DNA Utilization by Estuarine Microbial Populations.
Author Paul, J. H. ; DeFlaun, M. F. ; Jeffrey, W. H. ;
CORP Author University of South Florida, St. Petersburg. Dept. of Marine Science.;Environmental Research Lab., Gulf Breeze, FL.;National Science Foundation, Washington, DC.
Publisher c1988
Year Published 1988
Report Number NSF-OCE84-15605; EPA/600/J-89/164;
Stock Number PB90-129495
Additional Subjects Aquatic microbiology ; Genetics ; Deoxyribonucleic acids ; Ribonucleic acids ; Plankton ; Thymidines ; Reprints ; Plasmids ; DNA probes ; Competitive binding ; Structural genes ; Navobiocin ; Thymine
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB90-129495 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 03/10/1990
Collation 9p
Abstract
The mechanisms of utilization of DNA by estarine microbial populations has been investigated by competition experiments and DNA uptake studies. Deoxyribonucleoside monophosphates (dNMP's), thymidine, thymine, and RNA all completed with the uptake of radioactivity from (3H)DNA in 4 hour incubations. In fifteen minute incubations, dNMP's had no effect or stimulated (3H)DNA binding, depending on concentration. Uptake of radioactivity from (3H)DNA resulted in little accumulation of TCA-soluble intracellular radioactivity, and was inhibited by the DNA synthesis inhibitor novobiocin. Molecular fractionation studies indicated that some radioactivity from (3H)DNA appeared in the RNA (10 and 30% at 4 and 24 h respectively) and protein (approximately 3%) fractions. The ability for esturine microbial assemblages to transport gene sequences was investigated by plasmid uptake studies followed by molecular probing. (Copyright (c) 1988 American Society for Microbiology.)