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RECORD NUMBER: 39 OF 59

OLS Field Name OLS Field Data
Main Title Possible use of Alcaligenes paradoxus as a biological monitor /
Author Bradley, Donald V. ; Rogers, Robert D. ; McFarlane, James C.
Other Authors
Author Title of a Work
Rogers, Robert D.
McFarlane, James C.
CORP Author Environmental Monitoring and Support Lab., Las Vegas, NV. Monitoring Systems Research and Development Div.
Publisher Environomental Monitoring and Support Laboratory, Office of Research and Development Division, Environmental Monitoring and Support Laboratory,
Year Published 1979
Report Number EPA-600/3-79-048
Stock Number PB-297 942
OCLC Number 05289644
Subjects Environmental monitoring. ; Mercury. ; Cadmium. ; Soil pollution ; Pollution--Measurement
Additional Subjects Indicator species ; Bacteria ; Soils ; Soil microbiology ; Tritium ; Concentration(Composition) ; Cadmium ; Mercury(Metal) ; Lead(Metal) ; Hydrogen ; Cells(Biology) ; Oxidation ; Experimental data ; Growth ; Taxonomy ; Bioindicators ; Alcaligenes paradoxus ; Heavy metals
Internet Access
Description Access URL
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=91019KPB.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EHAM  TD196.C28B72 Region 1 Library/Boston,MA 04/29/2016
EJBD ARCHIVE EPA 600-3-79-048 Headquarters Library/Washington,DC 04/10/2014
EJED  EPA 600/3-79/048 OCSPP Chemical Library/Washington,DC 02/11/2005
EKAM  EPA/600/3-79/048 Region 4 Library/Atlanta,GA 05/13/1994
EKBD  EPA-600/3-79-048 Research Triangle Park Library/RTP, NC 08/11/2000
EKCD  EPA-600/3-79-048 CEMM/GEMMD Library/Gulf Breeze,FL 06/26/2018
ESAD  EPA 600-3-79-048 Region 10 Library/Seattle,WA 03/23/2010
NTIS  PB-297 942 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 01/01/1988
Collation 32 pages : illustrations ; 28 cm.
Abstract
A tritium (3H2)-oxidizing soil isolate was identified as Alcaligenes paradoxus, a gram-negative, rod-shaped bacterium. This organism belongs to a group of facultative autotrophs referred to as the 'hydrogen bacteria' due to their unique ability to utilize hydrogen as a sole source of energy for chemolithotrophic growth. Experiments with washed cells of A. paradoxus suspended in 0.025M potassium phosphate buffer (resting cells) showed that 1.0 ppm mercury (as Hg(NO3)2) caused a 95 percent reduction in hydrogen oxidation, whereas, concentration of 0.1 ppm and lower showed no inhibitory effects. When suspensions of A. paradoxus were added to sterile soil and then amended with mercury at concentrations of 1.0, 10.0, and 100.0 ppm, hydrogen oxidation was reduced 80 percent, 85 percent, and 95 percent, respectively, compared to soil controls with no mercury added. This showed that soil afforded, at least temporarily, some protection from toxic mercury effects. When cadmium or lead was used in solution, it was found that up to 100.0 ppm of these metals produced no detectable inhibition in the rate or extent of the hydrogen oxidation reaction at either pH 7.0 or 5.0. However, pretreatment of A. paradoxus cells with a combination of ethylenediaminetetraacetate (EDTA) and tris-(hydroxymethyl)-aminomethane (Tris), at a pH of 8.0, resulted in a reduction in oxidation in solutions containing 10.0 ppm of cadmium or lead, with the greater reduction being due to cadmium.
Notes
Includes bibliographical references (28-32).