Abstract |
The effect of free available chlorine upon test waters containing spores of Bacillus globigii and Bacillus anthracis was determined. Using contact periods of two hours and forty-eight hours, test waters containing free available chlorine and spores were evaluated at hydrogen-ion concentrations of 6.2, 7.2, 8.6 and 10.3-10.5 and storage temperatures of 4 and 22C. The amount of free available chlorine required to achieve a 99.99% kill under the above mentioned conditions was determined. Special procedures were developed for cultivation and standardization of spore suspensions, germination and sporulation media, and high quality synthetic buffered test waters. (Author) |