||Infection Intensity of 'Perkinsus marinus' Disease in 'Crassostrea virginica' (Gmelin, 1791) from the Gulf of Mexico Maintained under Different Laboratory Conditions.
Fisher, W. S. ;
Gauthier, J. D. ;
Winstead, J. T. ;
||Texas Univ. Medical Branch at Galveston. Marine Biomedical Inst.;Environmental Research Lab., Gulf Breeze, FL. Center for Marine and Estuarine Disease Research.;Texas A and M Univ. at Galveston. Sea Grant Coll. Program.
||NA89AA-D-SG139; EPA/600/J-93/057 ; CONTRIB-769
Protozoan infections ;
Mexico Gulf ;
Perkinsus marinus ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
A protozoan parasite, Perkinsus marinus, has been responsible for infection and mortality of eastern oysters, Crassostrea virginica, since before 1950. Studies on the course of infection intensity (incidence) in individual animals have been restricted by the need to sacrifice animals for diagnosis, so quantitative association of disease intensity with environmental conditions and individual survival has not been accomplished. A recently developed hemolymph assay provided the means to quantitate infection intensity from live oysters. Application of this technique demonstrated progression of P. marinus intensity in Gulf of Mexico oysters maintained in laboratory aquaria in fed and unfed conditions at different test temperatures (18 - 27 C) and salinities (6 - 36 ppt). In one experiment, incidences over eight weekly samplings were 10 (sup 0.09) hypnospores/ml hemolymph/week for low temperature/low salinity conditions and 10 (sup 0.36) hypnospores/ml hemolymph/week for high temperature/high salinity conditions. Temperature was more influential than salinity in P. marinus incidence and oyster mortalities.