Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Guidelines for conducting early life stage toxicity tests with Japanese Medaka 'oryzias latipes' {microfiche} /
Author Benoit, D. A. ; Holcombe, G. W. ; Spehar, R. L.
CORP Author Environmental Research Lab.-Duluth, MN.
Publisher Environmental Research Laboratory, US Environmental Protection Agency
Year Published 1991
Report Number EPA/600/3-91/063
Stock Number PB92-137488
Subjects Japanese Medaka ; 'oryzias latipes'
Additional Subjects Water pollution effects(Animals) ; Oryzias ; Life cycles ; Toxic substances ; Embryo ; Test methods ; Manuals ; Temperature ; Exposure ; Light ; Oxygen ; Guidelines
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
NTIS  PB92-137488 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. NTIS 01/01/1988
Collation 28 p.,: ill. ; 28 cm.
The manual represents a procedural guide for conducting embryo-larval early life stage (ELS) toxicity tests with Japanese medaka (Oryzias latipes). These procedures are based upon evaluation of published papers and recent methods development work conducted at a laboratory in Duluth. The published papers are referenced in the appropriate places throughout the text of the report. If more detailed information on test apparatus or specific biological and chemical methods is desired, the reader is encouraged to study the reference material or contact one of the authors of the manual. All routine methods not covered in the procedure (physical and chemical determinations), should be followed as described in Standard Methods for the Examination of Water and Wastewater (3). It is suggested that those routines dealing with handling of fish not covered in the procedure should be followed as described by ASTM (4).
"EPA 600/3-91/063". "December, 1991".